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Last updated: 2017/10/22
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EPAS1 polyclonal antibody

  • Catalog # : PAB12124
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of EPAS1.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human and mouse EPAS1/Epas1.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 118
  • Reactivity:
  • Human, Mouse, Primates, Rat
  • Specificity:
  • Specific for HIF-2 alpha/EPAS. Does not cross-react with HIF-1 alpha.
  • Form:
  • Liquid
  • Purity:
  • Immunogen affinity purification
  • Recommend Usage:
  • ChIP (1:10-1:500)
    ELISA (1:100-1:2000)
    Flow Cytometry
    Gel Supershift Assay
    Immunocytochemistry (1:100)
    Immunofluorescence (1:100)
    Immunohistochemistry (Frozen sections)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100)
    Immunoprecipitation
    Western Blot (1.0-2.0 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • ChIP
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of (1) Cobalt chloride treated COS-7 nuclear extracts and (2) untreated COS-7 nuclear extracts with EPAS1 polyclonal antibody (Cat # PAB12124).
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of (1) normoxic and (2) hypoxic nuclear rat cell lysates with EPAS1 polyclonal antibody (Cat # PAB12124).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cardiac myocytes with EPAS1 polyclonal antibody (Cat # PAB12124).
  • Immunohistochemistry (Frozen sections)
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of RCC4 cells with EPAS1 polyclonal antibody (Cat # PAB12124) at a 1:200 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.
  • Immunoprecipitation
  • ELISA
  • Gel Supershift Assay
  • Flow Cytometry
  • Application Image
  • ChIP
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunohistochemistry (Frozen sections)
  • Immunocytochemistry
  • Immunoprecipitation
  • ELISA
  • Gel Supershift Assay
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 2034
  • Gene Name:
  • EPAS1
  • Gene Alias:
  • ECYT4,HIF2A,HLF,MOP2,PASD2,bHLHe73
  • Gene Description:
  • endothelial PAS domain protein 1
  • Other Designations:
  • HIF-1alpha-like factor
  • Gene Information
  • Gene Name:
  • Epas1
  • Gene Alias:
  • HIF-2alpha,HLF,HRF,MOP2
  • Gene Description:
  • endothelial PAS domain protein 1
  • Other Designations:
  • HIF1 alpha-like factor,HIF1alpha-like factor,HLF (HIF1alpha-like factor),Hif like protein,hypoxia inducible transcription factor 2alpha
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