Lcat polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of plasma LCAT protein mass using Lcat polyclonal antibody (Cat # PAB12121). Plasma samples (0.2ul) from four hA-I Tg mice, four hA-I Tg SR-BI -/- mice, a LCAT-/- mouse, and purified mouse LCAT.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant Lcat.
Immunogen
Recombinant His fusion protein corresponding to C-terminus of mouse Lcat.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.05% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of plasma LCAT protein mass using Lcat polyclonal antibody (Cat # PAB12121). Plasma samples (0.2ul) from four hA-I Tg mice, four hA-I Tg SR-BI -/- mice, a LCAT-/- mouse, and purified mouse LCAT. -
Gene Info — Lcat
Entrez GeneID
16816Protein Accession#
P04180(human)Gene Name
Lcat
Gene Alias
AI046659, D8Wsu61e
Gene Description
lecithin cholesterol acyltransferase
Gene Ontology
HyperlinkOther Designations
lecithin-cholesterol acyltransferase Lcat
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Publication Reference
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LCAT synthesized by primary astrocytes esterifies cholesterol on glia-derived lipoproteins.
Hirsch-Reinshagen V, Donkin J, Stukas S, Chan J, Wilkinson A, Fan J, Parks JS, Kuivenhoven JA, Lutjohann D, Pritchard H, Wellington CL.
Journal of Lipid Research 2009 May; 50(5):885.
Application：WB, Mouse, Mouse brain.
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Initial interaction of apoA-I with ABCA1 impacts in vivo metabolic fate of nascent HDL.
Mulya A, Lee JY, Gebre AK, Boudyguina EY, Chung SK, Smith TL, Colvin PL, Jiang XC, Parks JS.
Journal of Lipid Research 2008 Nov; 49(11):2390.
Application：Func, Mouse, Mouse plasma.
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Functional LCAT deficiency in human apolipoprotein A-I transgenic, SR-BI knockout mice.
Lee JY, Badeau RM, Mulya A, Boudyguina E, Gebre AK, Smith TL, Parks JS.
Journal of Lipid Research 2007 May; 48(5):1052.
Application：WB, Mouse , Mouse plasma.
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In vivo contribution of LCAT to apolipoprotein B lipoprotein cholesteryl esters in LDL receptor and apolipoprotein E knockout mice.
Furbee JW Jr, Francone O, Parks JS.
Journal of Lipid Research 2002 Mar; 43(3):428.
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Lecithin:cholesterol acyltransferase deficiency increases atherosclerosis in the low density lipoprotein receptor and apolipoprotein E knockout mice.
Furbee JW Jr, Sawyer JK, Parks JS.
The Journal of Biological Chemistry 2002 Feb; 277(5):3511.
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LCAT synthesized by primary astrocytes esterifies cholesterol on glia-derived lipoproteins.
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