Nefl polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against native Nefl.
Immunogen
Native purified Nefl from pig spinal cord.
Host
Rabbit
Theoretical MW (kDa)
68
Reactivity
Human, Mouse, Rat
Specificity
Specifically recognizes the light neurofilament subunit (~68 KDa).
Form
Liquid
Purity
Unpurified
Recommend Usage
Immunocytochemistry/Immunofluorescence (1:500-1:5000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:5000)
Western Blot (1:10000-1:15000)
The optimal working dilution should be determined by the end user.Storage Buffer
In whole antisera (5 mM sodium azide).
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of whole rat cerebellum homogenate stained with Nefl polyclonal antibody (Cat # PAB12120), at dilution of 1 : 20,000.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
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Gene Info — Nefl
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