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Last updated: 2016/12/4
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Nefh polyclonal antibody

  • Catalog # : PAB12117
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial recombinant Nefh.
  • Immunogen:
  • Recombinant protein corresponding to C-terminus of rat Nefh.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 180-220
  • Reactivity:
  • Human, Mammals, Mouse, Primates, Rat
  • Specificity:
  • This antibody specifically recognizes the heavy microfilament subunit (~180-220 KDa).
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In antiserum (0.05% sodium azide)
  • Storage Instruction:
  • Store at 4°C for short term. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Frozen sections)
  • Immunofluorescence
  • Immunofluorescence
  • Section of rat spinal cord stained with Nefh polyclonal antibody (Cat # PAB12117) (green) and monoclonal antibody to ubiquitin C-terminal hydrolase 1. The Nefh antibody binds primarily to phosphorylated axonal forms of NF-H, and so stains axons coursing between the large UCHL1 positive neurons. These large cells are a-motorneurons and UCHL1 protein is a major component of the perikarya and dendrites of these cells.
  • Application Image
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Frozen sections)
  • Gene Information
  • Gene Name:
  • Nefh
  • Gene Alias:
  • Nfh
  • Gene Description:
  • neurofilament, heavy polypeptide
  • Other Designations:
  • -
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