Rabbit polyclonal antibody raised against synthetic peptide of Aqp2.
Immunogen:
A synthetic peptide corresponding to residues within amino acids 200-300 at C-terminus of rat Aqp2.
Host:
Rabbit
Reactivity:
Human, Mouse, Rat
Form:
Liquid
Purification:
Immunogen affinity purification
Recommend Usage:
Immunocytochemistry/Immunofluorescence (1 ug/mL) Immunohistochemistry (1:100-1:200) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100-1:200) Western Blot (1-2 ug/mL) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (0.05% sodium azide).
Storage Instruction:
Store at 4°C for short term. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. This antibody is useful for western blot, where bands can be seen ~28 kDa for the non-glycosylated form and ~37 kDa for the glycosylated form.
Western blot analysis of Aqp2 in rat kidney extracts using Aqp2 polyclonal antibody (Cat # PAB12114). Key : CX=Cortex, OM=Outer Medulla, IMB =Inner Medulla Base, IMP = Inner Medulla Papilla.