Gap43 polyclonal antibody
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More Files
- More Functions
Immunofluorescence
Immunofluorescence of GAP43 (green) demonstrates intense staining in overexpressing wild-type PS-1 (E) PC-12 cells using Gap43 polyclonal antibody (Cat # PAB12102). (Teo, et al, 2005) .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Gap43.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 216-226 of mouse/rat Gap43.
Sequence
KEDPEADQEHA
Host
Rabbit
Reactivity
Chicken, Human, Mouse, Rat
Specificity
This antibody is specific to GAP-43.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL)
Immunohistochemistry (Frozen sections) (1-2 ug/mL)
Immunofluorescence (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunofluorescence
Immunofluorescence of GAP43 (green) demonstrates intense staining in overexpressing wild-type PS-1 (E) PC-12 cells using Gap43 polyclonal antibody (Cat # PAB12102). (Teo, et al, 2005) . -
Gene Info — Gap43
Entrez GeneID
14432Protein Accession#
P07936Gene Name
Gap43
Gene Alias
B-50, Basp2, GAP-43
Gene Description
growth associated protein 43
Gene Ontology
HyperlinkGene Summary
membrane attached signal protein 2|growth accentuating protein 43|neuromodulin
Other Designations
OTTMUSP00000017444|brain abundant, membrane attached signal protein 2|growth accentuating protein 43|neuromodulin
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Gene Info — Gap43
Entrez GeneID
29423Protein Accession#
P07936Gene Name
Gap43
Gene Alias
Basp2, MGC156666
Gene Description
growth associated protein 43
Gene Ontology
HyperlinkGene Summary
membrane attached signal protein 2|growth accentuating protein 43
Other Designations
brain abundant, membrane attached signal protein 2|growth accentuating protein 43
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Publication Reference
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Interaction of amyloid precursor protein with contactins and NgCAM in the retinotectal system.
Osterfield M, Egelund R, Young LM, Flanagan JG.
Development 2008 Mar; 135(6):1189.
Application：IF, IHC, Chicken, Sagittal sections of E11.5 chick heads.
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Metalloproteinase-dependent predegeneration in vitro enhances axonal regeneration within acellular peripheral nerve grafts.
Krekoski CA, Neubauer D, Graham JB, Muir D.
The Journal of Neuroscience 2002 Dec; 22(23):10408.
Application：IF, Rat, Nerve explant culture.
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Regeneration of axons after nerve transection repair is enhanced by degradation of chondroitin sulfate proteoglycan.
Zuo J, Neubauer D, Graham J, Krekoski CA, Ferguson TA, Muir D.
Experimental Neurology 2002 Jul; 176(1):221.
Application：ICC, Rat, Sciatic nerve.
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Axonal regeneration into acellular nerve grafts is enhanced by degradation of chondroitin sulfate proteoglycan.
Krekoski CA, Neubauer D, Zuo J, Muir D.
Journal of Neuroscience 2001 Aug; 21(16):6206.
Application：ICC, IF, IHC-Fr, Rat, Rat sciatic nerves.
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MMP-2 and MMP-9 increase the neurite-promoting potential of schwann cell basal laminae and are upregulated in degenerated nerve.
Ferguson TA, Muir D.
Molecular and Cellular Neurosciences 2000 Aug; 16(2):157.
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Interaction of amyloid precursor protein with contactins and NgCAM in the retinotectal system.
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