Rabbit polyclonal antibody raised against synthetic peptide of NOX4.
A synthetic peptide corresponding to amino acids 100-200 of human NOX4.
Bovine, Human, Mouse, Primates, Rat, Sheep
Immunocytochemistry (1:50-1:200) Immunofluorescence (1:50-1:200) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5 ug/mL) Western Blot (2 ug/mL) The optimal working dilution should be determined by the end user.
In Tris-citrate/phosphate buffer, pH 7.0-8.0 (0.09% sodium azide)
Store at 4°C. Do not freeze.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of NOX4 in human kidney lysates with NOX4 polyclonal antibody at 2.0 ug/mL (Cat # PAB12095). A non-specific band is often observed running at 50 kDa in tissue lysates which is believed to correspond to the human IgG heavy chain.
This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants