Npc1l1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Npc1l1.
Immunogen
A synthetic peptide corresponding to amino acids 500-600 of rat Npc1l1.
Host
Rabbit
Reactivity
Mouse, Rat
Specificity
This antibody is specific to NPC1L1 protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunohistochemistry (1 ug/mL)
Western Blot (1:500)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris-citrate/phosphate buffer, pH 7.0-8.0
Storage Instruction
Store at 4°C. Do not freeze.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of Npc1l1 in rat small intestine membrane preparations (Lane 1 : 2 ug/mL , Lane 2 : 4 ug/mL) with Npc1l1 polyclonal antibody (Cat # PAB12091).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry
Immunohistochemical staining of Npc1l1 on mouse intestine with Npc1l1 polyclonal antibody (Cat # PAB12091). -
Gene Info — Npc1l1
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Publication Reference
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Niemann-Pick C1 functions in regulating lysosomal amine content.
Kaufmann AM, Krise JP.
The Journal of Biological Chemistry 2008 Sep; 283(36):24584.
Application:IF, WB-Tr, Human, Normal human fibroblasts.
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Intestinal fatty acid binding protein regulates mitochondrion beta-oxidation and cholesterol uptake.
Montoudis A, Seidman E, Boudreau F, Beaulieu JF, Menard D, Elchebly M, Mailhot G, Sane AT, Lambert M, Delvin E, Levy E.
Journal of Lipid Research 2008 May; 49(5):961.
Application:WB-Ce, Human, Normal human intestinal epithelial cells.
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Niemann-Pick C1 Like 1 protein is critical for intestinal cholesterol absorption.
Altmann SW, Davis HR Jr, Zhu LJ, Yao X, Hoos LM, Tetzloff G, Iyer SP, Maguire M, Golovko A, Zeng M, Wang L, Murgolo N, Graziano MP.
Science 2004 Feb; 303(5661):1201.
Application:IHC, WB, Mouse Rat, Mouse jejunal enterocytes, Rat jejunum, Rat small intestine.
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Niemann-Pick C1 functions in regulating lysosomal amine content.
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