NTHL1 polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NTHL1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to human NTHL1.
Host
Rabbit
Reactivity
Bovine, Human, Rat
Specificity
This antibody is specific to NTH1.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunocytochemistry (1:800)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of NTHL1 in HeLa (Lane2) and K-562 (Lane1) cell extracts with NTHL1 polyclonal antibody (Cat # PAB12089). Western blot data courtesy of Mark Kelly, Indiana Univ.Immunocytochemistry
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Gene Info — NTHL1
Entrez GeneID
4913Gene Name
NTHL1
Gene Alias
NTH1, OCTS3
Gene Description
nth endonuclease III-like 1 (E. coli)
Omim ID
602656Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a DNA N-glycosylase of the endonuclease III family. Like a similar protein in E. coli, the encoded protein has DNA glycosylase activity on DNA substrates containing oxidized pyrimidine residues and has apurinic/apyrimidinic lyase activity. [provided by RefSeq
Other Designations
OTTHUMP00000158939|nth endonuclease III-like 1
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Interactomes
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Pathways
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Diseases
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Publication Reference
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p21(Cip1/WAF1/Sdi1) does not affect expression of base excision DNA repair enzymes during chronic oxidative stress.
O'reilly MA, Vitiello PF, Gehen SC, Staversky RJ.
Antioxidants & Redox Signaling 2005 May; 7(5-6):719.
Application:WB, Human, Mouse, H1299 cells, Mouse lungs.
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Cloning and characterization of a mouse homologue (mNthl1) of Escherichia coli endonuclease III.
Sarker AH, Ikeda S, Nakano H, Terato H, Ide H, Imai K, Akiyama K, Tsutsui K, Bo Z, Kubo K, Yamamoto K, Yasui A, Yoshida MC, Seki S.
Journal of Molecular Biology 1998 Oct; 282(4):761.
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Mitochondrial targeting of human DNA glycosylases for repair of oxidative DNA damage.
Takao M, Aburatani H, Kobayashi K, Yasui A.
Nucleic Acids Research 1998 Jun; 26(12):2917.
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p21(Cip1/WAF1/Sdi1) does not affect expression of base excision DNA repair enzymes during chronic oxidative stress.
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