PDHA1 (phospho S293) polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot
Western blot analysis of PDHA1 in an in vitro autophosphorylation of PDH complex in response to different stimulants with PDHA1 (phospho S293) polyclonal antibody (Cat # PAB12079).
Such stimulants are : (-) none, H2O2-hydrogen peroxide, AR-anhydroretinol, Rol-retinol, RA-retinoic acid, PMA, Lipid-PKC lipid activator.
Photo courtesy of Dr. Beatrice Hoyos, Memorial Sloan-Kettering Cancer Center.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of PDHA1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S293 human PDHA1.
Host
Rabbit
Reactivity
Human
Specificity
This antibody is specific to the phosphorylated Serine 293 form of the PDHE1 alpha protein.
Form
Liquid
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris-citrate/phosphate buffer, pH 7.0-8.0 (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot
Western blot analysis of PDHA1 in an in vitro autophosphorylation of PDH complex in response to different stimulants with PDHA1 (phospho S293) polyclonal antibody (Cat # PAB12079).
Such stimulants are : (-) none, H2O2-hydrogen peroxide, AR-anhydroretinol, Rol-retinol, RA-retinoic acid, PMA, Lipid-PKC lipid activator.
Photo courtesy of Dr. Beatrice Hoyos, Memorial Sloan-Kettering Cancer Center.Immunocytochemistry
Immunofluorescence
-
Gene Info — PDHA1
Entrez GeneID
5160Protein Accession#
P08559Gene Name
PDHA1
Gene Alias
PDHA, PDHCE1A, PHE1A
Gene Description
pyruvate dehydrogenase (lipoamide) alpha 1
Gene Ontology
HyperlinkGene Summary
The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT; MIM 608770) (E2; EC 2.3.1.12); and dihydrolipoyl dehydrogenase (DLD; MIM 238331) (E3; EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM
Other Designations
OTTHUMP00000023015|pyruvate dehydrogenase E1 alpha subunit
-
Interactome
-
Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Butanoate metabolism
- Citrate cycle (TCA cycle)
+ View More Disease
-
Publication Reference
-
Pyruvate dehydrogenase complex activity controls metabolic and malignant phenotype in cancer cells.
McFate T, Mohyeldin A, Lu H, Thakar J, Henriques J, Halim ND, Wu H, Schell MJ, Tsang TM, Teahan O, Zhou S, Califano JA, Jeoung NH, Harris RA, Verma A.
The Journal of Biological Chemistry 2008 Jun; 283(33):22700.
-
Pyruvate dehydrogenase complex activity controls metabolic and malignant phenotype in cancer cells.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com