POLI polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of POLI.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human POLI.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody is specific to human DNA Polymerase Iota.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.02% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
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Gene Info — POLI
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Interactome
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Disease
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Publication Reference
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Human DNA polymerase iota promiscuous mismatch extension.
Vaisman A, Tissier A, Frank EG, Goodman MF, Woodgate R.
The Journal of Biological Chemistry 2001 Aug; 276(33):30615.
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Altered nucleotide misinsertion fidelity associated with poliota-dependent replication at the end of a DNA template.
Frank EG, Tissier A, McDonald JP, Rapic-Otrin V, Zeng X, Gearhart PJ, Woodgate R.
The EMBO Journal 2001 Jun; 20(11):2914.
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Misinsertion and bypass of thymine-thymine dimers by human DNA polymerase iota.
Tissier A, Frank EG, McDonald JP, Iwai S, Hanaoka F, Woodgate R.
The EMBO Journal 2000 Oct; 19(19):5259.
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Human DNA polymerase iota promiscuous mismatch extension.
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