RAD50 polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of RAD50 in 25 ng purified hMre11-hRAD50 complex (Lane 1) and 25 ug crude HeLa extract (Lane 2 :) with RAD50 polyclonal antibody (Cat # PAB12052).
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant RAD50.
Immunogen
Recombinant protein corresponding to amino acids 628-787 of human RAD50.
Host
Rabbit
Reactivity
Human
Specificity
This antibody is specific to human Rad50.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western Blot (1:1000-1:10000)
Immunoprecipitation (1-5 ug/mL lysate)
The optimal working dilution should be determined by the end user.Storage Buffer
No additive
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot analysis of RAD50 in 25 ng purified hMre11-hRAD50 complex (Lane 1) and 25 ug crude HeLa extract (Lane 2 :) with RAD50 polyclonal antibody (Cat # PAB12052).Immunoprecipitation
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Gene Info — RAD50
Entrez GeneID
10111Gene Name
RAD50
Gene Alias
RAD50-2, hRad50
Gene Description
RAD50 homolog (S. cerevisiae)
Omim ID
604040Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq
Other Designations
OTTHUMP00000065928|RAD50 homolog
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Interactome
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Pathway
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Disease
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Publication Reference
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Dimerization of the Rad50 protein is independent of the conserved hook domain.
Cahill D, Carney JP.
Mutagenesis 2007 Jul; 22(4):269.
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The Rad50 zinc-hook is a structure joining Mre11 complexes in DNA recombination and repair.
Hopfner KP, Craig L, Moncalian G, Zinkel RA, Usui T, Owen BA, Karcher A, Henderson B, Bodmer JL, McMurray CT, Carney JP, Petrini JH, Tainer JA.
Nature 2002 Aug; 418(6897):562.
Application：WB-Ce, Yeast, Yeast.
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The mammalian Mre11-Rad50-nbs1 protein complex: integration of functions in the cellular DNA-damage response.
Petrini JH.
American Journal of Human Genetics 1999 May; 64(5):1264.
Application：IHC, WB-Ce, WB-Tr, Human, Cancers, Mammalian cells.
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Dimerization of the Rad50 protein is independent of the conserved hook domain.
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