Rabbit polyclonal antibody raised against partial recombinant SPTBN2.
Recombinant protein corresponding to C-terminus of human SPTBN2.
The epitope recognized by this antibody maps to a C-terminal region between residue 2300 and 2390 of human spectrin, beta, non-erythrocytic 2 (spinocerebellar ataxia 5 ) using the numbering given in entry NP_008877.1
Quality Control Testing:
Antibody Reactive Against Recombinant Protein.
Immunohistochemistry (10-20 ug/mL) Western Blot (1:2000-1:10000) The optimal working dilution should be determined by the end user.
In Tris-citrate/phosphate buffer, pH 7.0-8.0 (0.09% sodium azide)
Store at 4°C. Do not freeze.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of SPTBN2 in HeLa whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK 293T (Bosc 23) (T : 50 ug) cells with SPTBN2 polyclonal antibody (Cat # PAB12022). Antibodies : SPTBN2 polyclonal antibody (Cat # PAB12022) used for WB at 0.04 ug/mL (A) and 1 ug/mL (B) and used for IP at 3 ug/mg lysate. SPTBN2 was also immunoprecipitated by SPTBN2 polyclonal antibody (Cat # PAB12023), which recognizes an upstream epitope. Detection : Chemiluminescence with exposure times of 10 seconds (A) and 30 seconds (B).