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Last updated: 2017/4/23
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SPTBN2 polyclonal antibody

  • Catalog # : PAB12022
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial recombinant SPTBN2.
  • Immunogen:
  • Recombinant protein corresponding to C-terminus of human SPTBN2.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • The epitope recognized by this antibody maps to a C-terminal region between residue 2300 and 2390 of human spectrin, beta, non-erythrocytic 2 (spinocerebellar ataxia 5 ) using the numbering given in entry NP_008877.1
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • Immunohistochemistry (10-20 ug/mL)
    Western Blot (1:2000-1:10000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris-citrate/phosphate buffer, pH 7.0-8.0 (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. Do not freeze.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of SPTBN2 in HeLa whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK 293T (Bosc 23) (T : 50 ug) cells with SPTBN2 polyclonal antibody (Cat # PAB12022).
    Antibodies : SPTBN2 polyclonal antibody (Cat # PAB12022) used for WB at 0.04 ug/mL (A) and 1 ug/mL (B) and used for IP at 3 ug/mg lysate.
    SPTBN2 was also immunoprecipitated by SPTBN2 polyclonal antibody (Cat # PAB12023), which recognizes an upstream epitope.
    Detection : Chemiluminescence with exposure times of 10 seconds (A) and 30 seconds (B).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry
  • Immunohistochemistry
  • Immunohistochemical staining of SPTBN2 in human prostate epithelium with SPTBN2 polyclonal antibody (Cat # PAB12022).
  • Immunoprecipitation
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 6712
  • Gene Name:
  • SPTBN2
  • Gene Alias:
  • GTRAP41,SCA5
  • Gene Description:
  • spectrin, beta, non-erythrocytic 2
  • Other Designations:
  • beta-III spectrin,spinocerebellar ataxia 5
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