RAD51 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of RAD51 polyclonal antibody (Cat # PAB1184) in HeLa cell extract.
Immunocytochemistry
Detection of RAD51 foc : formation induced by DNA damage.
Normal human diploid cells were irradiated by X-ray (0.5 Gly) and after incubation of 6 hrs.
The cells were fixed and immuno-stained by using RAD51 polyclonal antibody (Cat # PAB1184) (100 dilution) as the primary antibody and Alexa594 labeled anti-rabbit antibody as the secondary antibody.
(The picture was Kindry prorided by Prof. K. Suzuki, Medical school of Nagasaki Univ.)
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant RAD51.
Immunogen
Recombinant protein corresponding to full length human RAD51.
Host
Rabbit
Reactivity
Chicken, Human, Mouse
Form
Liquid
Recommend Usage
Western Blot (1:2000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In buffer containing 0.09% sodium azide
Storage Instruction
Store the kit at -20°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of RAD51 polyclonal antibody (Cat # PAB1184) in HeLa cell extract.Immunocytochemistry
Detection of RAD51 foc : formation induced by DNA damage.
Normal human diploid cells were irradiated by X-ray (0.5 Gly) and after incubation of 6 hrs.
The cells were fixed and immuno-stained by using RAD51 polyclonal antibody (Cat # PAB1184) (100 dilution) as the primary antibody and Alexa594 labeled anti-rabbit antibody as the secondary antibody.
(The picture was Kindry prorided by Prof. K. Suzuki, Medical school of Nagasaki Univ.)Immunofluorescence
Immunoprecipitation
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Gene Info — RAD51
Entrez GeneID
5888Gene Name
RAD51
Gene Alias
BRCC5, HRAD51, HsRad51, HsT16930, RAD51A, RECA
Gene Description
RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
BRCA1/BRCA2-containing complex, subunit 5|DNA repair protein RAD51 homolog 1|RAD51 homolog protein|RAD51, S.cerevisiae, homolog of, A|RecA, E. coli, homolog of|RecA-like protein|recombination protein A
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Interactome
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Pathway
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Disease
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