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Last updated: 2016/11/27
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RAD51 polyclonal antibody

  • Catalog # : PAB1184
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against full length recombinant RAD51.
  • Immunogen:
  • Recombinant protein corresponding to full length human RAD51.
  • Host:
  • Rabbit
  • Reactivity:
  • Chicken, Human, Mouse
  • Form:
  • Liquid
  • Recommend Usage:
  • Western Blot (1:2000-1:10000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In buffer containing 0.09% sodium azide
  • Storage Instruction:
  • Store the kit at -20°C.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of RAD51 polyclonal antibody (Cat # PAB1184) in HeLa cell extract.
  • Immunocytochemistry
  • Immunocytochemistry
  • Detection of RAD51 foc : formation induced by DNA damage.
    Normal human diploid cells were irradiated by X-ray (0.5 Gly) and after incubation of 6 hrs.
    The cells were fixed and immuno-stained by using RAD51 polyclonal antibody (Cat # PAB1184) (100 dilution) as the primary antibody and Alexa594 labeled anti-rabbit antibody as the secondary antibody.
    (The picture was Kindry prorided by Prof. K. Suzuki, Medical school of Nagasaki Univ.)
  • Immunofluorescence
  • Immunoprecipitation
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunofluorescence
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 5888
  • Gene Name:
  • RAD51
  • Gene Alias:
  • BRCC5,HRAD51,HsRad51,HsT16930,RAD51A,RECA
  • Gene Description:
  • RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
  • Gene Summary:
  • The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • BRCA1/BRCA2-containing complex, subunit 5,DNA repair protein RAD51 homolog 1,RAD51 homolog protein,RAD51, S.cerevisiae, homolog of, A,RecA, E. coli, homolog of,RecA-like protein,recombination protein A
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