NDEL1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NDEL1.
Immunogen
A synthetic peptide corresponding to human NDEL1.
Sequence
CRNFAKDQASRKSY
Host
Goat
Theoretical MW (kDa)
37, 38.4
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:8000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
NDEL1 polyclonal antibody (Cat # PAB11614) (0.1 ug/mL) staining of human cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NDEL1
Entrez GeneID
81565Protein Accession#
NP_001020750.1;NP_110435.1Gene Name
NDEL1
Gene Alias
DKFZp451M0318, EOPA, MITAP1, NUDEL
Gene Description
nudE nuclear distribution gene E homolog (A. nidulans)-like 1
Omim ID
607538Gene Ontology
HyperlinkGene Summary
This gene encodes a thiol-activated peptidase that is phosphorylated in M phase of the cell cycle. Phosphorylation regulates the cell cycle-dependent distribution of this protein, with a fraction of the protein bound strongly to centrosomes in interphase and localized to mitotic spindles in early M phase. Overall, this protein plays a role in nervous system development. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
NUDE-like protein|endooligopeptidase A|mitosin-associated protein MITAP1|nudE nuclear distribution gene E homolog like 1
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Interactome
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Disease
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Publication Reference
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Neuroepithelial stem cell proliferation requires LIS1 for precise spindle orientation and symmetric division.
Yingling J, Youn YH, Darling D, Toyo-Oka K, Pramparo T, Hirotsune S, Wynshaw-Boris A.
Cell 2008 Feb; 132(3):474.
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Neuroepithelial stem cell proliferation requires LIS1 for precise spindle orientation and symmetric division.
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