MED15 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MED15.
Immunogen
A synthetic peptide corresponding to human MED15.
Sequence
C-PPQSQTQPLVSQAQ
Host
Goat
Theoretical MW (kDa)
86.8, 82.6
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:8000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
MED15 polyclonal antibody (Cat # PAB11613) (1 ug/mL) staining of human lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MED15
Entrez GeneID
51586Protein Accession#
NP_001003891.1;NP_056973.2Gene Name
MED15
Gene Alias
ARC105, CAG7A, CTG7A, DKFZp686A2214, DKFZp762B1216, FLJ42282, FLJ42935, PCQAP, TIG-1, TIG1, TNRC7
Gene Description
mediator complex subunit 15
Omim ID
607372Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
CTG repeat protein 7a|PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associated protein|PC2-glutamine-rich-associated protein|TPA inducible gene-1|TPA inducible protein|activator-recruited cofactor, 105-kD|positive cofactor 2, glutamine/
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Interactome
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Disease
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Publication Reference
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A nuclear receptor-like pathway regulating multidrug resistance in fungi.
Thakur JK, Arthanari H, Yang F, Pan SJ, Fan X, Breger J, Frueh DP, Gulshan K, Li DK, Mylonakis E, Struhl K, Moye-Rowley WS, Cormack BP, Wagner G, Naar AM.
Nature 2008 Apr; 452(7187):604.
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A nuclear receptor-like pathway regulating multidrug resistance in fungi.
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