TRPC4 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of TRPC4.
Immunogen
A synthetic peptide corresponding to human TRPC4.
Sequence
C-KHAKEEDSSIDYD
Host
Goat
Theoretical MW (kDa)
112
Reactivity
Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
TRPC4 polyclonal antibody (Cat # PAB11598) (0.3 ug/mL) staining of rat brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — TRPC4
Entrez GeneID
7223Protein Accession#
NP_057263.1Gene Name
TRPC4
Gene Alias
HTRP4, MGC119570, MGC119571, MGC119572, MGC119573, TRP4
Gene Description
transient receptor potential cation channel, subfamily C, member 4
Omim ID
603651Gene Ontology
HyperlinkGene Summary
subfamily C
Other Designations
OTTHUMP00000018272|transient receptor potential 4
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Interactome
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Disease
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Publication Reference
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Impaired Orai1-mediated Resting Ca2+ Entry Reduces the Cytosolic [Ca2+] and Sarcoplasmic Reticulum Ca2+ Loading in Quiescent Junctophilin 1 Knock-out Myotubes.
Li H, Ding X, Lopez JR, Takeshima H, Ma J, Allen PD, Eltit JM.
The Journal of Biological Chemistry 2010 Dec; 285(50):39171.
Application:WB, Human, Myotube.
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Canonical transient receptor potential channel 4 (TRPC4) co-localizes with the scaffolding protein ZO-1 in human fetal astrocytes in culture.
Song X, Zhao Y, Narcisse L, Duffy H, Kress Y, Lee S, Brosnan CF.
Glia 2005 Feb; 49(3):418.
Application:ICC, IEM, IP, WB, Human, Human fetal astrocytes.
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Impaired Orai1-mediated Resting Ca2+ Entry Reduces the Cytosolic [Ca2+] and Sarcoplasmic Reticulum Ca2+ Loading in Quiescent Junctophilin 1 Knock-out Myotubes.
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