Ramp1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of Ramp1.
Immunogen
A synthetic peptide corresponding to mouse Ramp1.
Sequence
C-DYQSKRTEGIV
Host
Goat
Theoretical MW (kDa)
16.9, 11.5
Reactivity
Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Ramp1 polyclonal antibody (Cat # PAB11575) (0.3 ug/mL) staining of rat brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Ramp1
Entrez GeneID
51801Protein Accession#
NP_058590.1;NP_848488.2Gene Name
Ramp1
Gene Alias
9130218E19Rik, MGC38240
Gene Description
receptor (calcitonin) activity modifying protein 1
Gene Ontology
HyperlinkOther Designations
receptor-activity modifying protein 1
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Publication Reference
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Hypertension and dysregulated proinflammatory cytokine production in receptor activity-modifying protein 1-deficient mice.
Tsujikawa K, Yayama K, Hayashi T, Matsushita H, Yamaguchi T, Shigeno T, Ogitani Y, Hirayama M, Kato T, Fukada S, Takatori S, Kawasaki H, Okamoto H, Ikawa M, Okabe M, Yamamoto H.
PNAS 2007 Oct; 104(42):16702.
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Agonist-promoted internalization of a ternary complex between calcitonin receptor-like receptor, receptor activity-modifying protein 1 (RAMP1), and beta-arrestin.
Hilairet S, Bélanger C, Bertrand J, Laperrière A, Foord SM, Bouvier M.
The Journal of Biological Chemistry 2001 Nov; 276(45):42182.
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Hypertension and dysregulated proinflammatory cytokine production in receptor activity-modifying protein 1-deficient mice.
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