PRUNE2 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PRUNE2.
Immunogen
A synthetic peptide corresponding to human PRUNE2.
Sequence
C-MESEKISEKQEE
Host
Goat
Theoretical MW (kDa)
29.5
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PRUNE2 polyclonal antibody (Cat # PAB11573) (1 ug/mL) staining of human brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRUNE2
Entrez GeneID
158471Protein Accession#
NP_620173.1Gene Name
PRUNE2
Gene Alias
A214N16.3, BMCC1, BNIPXL, C9orf65, DKFZp762K117, FLJ50060, FLJ54876, FLJ59118, KIAA0367, RP11-58J3.2, bA214N16.3
Gene Description
prune homolog 2 (Drosophila)
Omim ID
610691Gene Ontology
HyperlinkOther Designations
BCH motif-containing molecule at the carboxyl terminal region 1|BNIP2 motif containing molecule at the carboxyl terminal region 1|OTTHUMP00000021508|prune homolog 2
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Disease
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Publication Reference
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Glycosylation-related gene expression is linked to differentiation status in glioblastomas undifferentiated cells.
Cheray M, Petit D, Forestier L, Karayan-Tapon L, Maftah A, Jauberteau MO, Battu S, Gallet FP, Lalloue F.
Cancer Letters 2011 Dec; 312(1):24.
Application:WB-Ce, Human, U87 MG, U251 cells.
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Increased expression of proapoptotic BMCC1, a novel gene with the BNIP2 and Cdc42GAP homology (BCH) domain, is associated with favorable prognosis in human neuroblastomas.
Machida T, Fujita T, Ooo ML, Ohira M, Isogai E, Mihara M, Hirato J, Tomotsune D, Hirata T, Fujimori M, Adachi W, Nakagawara A.
Oncogene 2006 Mar; 25(13):1931.
Application:IHC, WB-Tr, Human, COS7, COS7-pCAGGS-BMCC1, HEK 293, HEK 293-pCAGGS-BMCC1 cells, Neuroblastomas.
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Glycosylation-related gene expression is linked to differentiation status in glioblastomas undifferentiated cells.
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