GCC2 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of GCC2.
Immunogen
A synthetic peptide corresponding to internal region of human GCC2.
Sequence
C-QKLTNKNNKIED
Host
Goat
Theoretical MW (kDa)
185, 196
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
GCC2 polyclonal antibody (Cat # PAB11531)(3.8 ug/mL) staining of paraffin embedded human spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GCC2
Entrez GeneID
9648Protein Accession#
NP_055450.1; NP_852118.1Gene Name
GCC2
Gene Alias
GCC185, KIAA0336
Gene Description
GRIP and coiled-coil domain containing 2
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a peripheral membrane protein localized to the trans-Golgi network. It is sensitive to brefeldin A. This encoded protein contains a GRIP domain which is thought to be used in targeting. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
CLL-associated antigen KW-11|CTCL tumor antigen se1-1|GRIP and coiled-coil domain-containing 2|GRIP coiled-coil protein GCC185
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Interactome
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Publication Reference
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Asymmetric CLASP-dependent nucleation of noncentrosomal microtubules at the trans-Golgi network.
Efimov A, Kharitonov A, Efimova N, Loncarek J, Miller PM, Andreyeva N, Gleeson P, Galjart N, Maia AR, McLeod IX, Yates JR 3rd, Maiato H, Khodjakov A, Akhmanova A, Kaverina I.
Developmental Cell 2007 Jun; 12(6):917.
Application:WB-Tr, Human, RPE1 cells.
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Asymmetric CLASP-dependent nucleation of noncentrosomal microtubules at the trans-Golgi network.
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