APOF polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
APOF polyclonal antibody (Cat # PAB11492) (0.5 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
APOF polyclonal antibody (Cat # PAB11492) (3.8 ug/mL) staining of paraffin embedded human liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of APOF.
Immunogen
A synthetic peptide corresponding to C-terminus of human APOF.
Sequence
C-KSYDLDPGAGSLE
Host
Goat
Theoretical MW (kDa)
35.4
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (0.3-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2.5-3.8 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
APOF polyclonal antibody (Cat # PAB11492) (0.5 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
APOF polyclonal antibody (Cat # PAB11492) (3.8 ug/mL) staining of paraffin embedded human liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — APOF
Entrez GeneID
319Protein Accession#
NP_001629.1Gene Name
APOF
Gene Alias
Apo-F, DKFZp781G18150, LTIP, MGC22520
Gene Description
apolipoprotein F
Omim ID
107760Gene Ontology
HyperlinkGene Summary
The product of this gene is one of the minor apolipoproteins found in plasma. This protein forms complexes with lipoproteins and may be involved in transport and/or esterification of cholesterol. [provided by RefSeq
Other Designations
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Interactome
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Disease
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Publication Reference
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Molecular cloning and expression of lipid transfer inhibitor protein reveals its identity with apolipoprotein F.
Wang X, Driscoll DM, Morton RE.
The Journal of Biological Chemistry 1999 Jan; 274(3):1814.
Application：WB, WB-Tr, Human, Monkey, COS-7 cells, Human plasma.
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Molecular cloning and expression of lipid transfer inhibitor protein reveals its identity with apolipoprotein F.
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