ABCA9 polyclonal antibody
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ABCA9 polyclonal antibody (Cat # PAB11478) (5 ug/mL) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry
ABCA9 polyclonal antibody (Cat # PAB11478) Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ABCA9.
Immunogen
A synthetic peptide corresponding to human ABCA9.
Sequence
C-QRVVQELEMENIQD
Host
Goat
Theoretical MW (kDa)
184
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Flow Cytometry (10 ug/mL)
Immunohistochemistry (5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ABCA9 polyclonal antibody (Cat # PAB11478) (5 ug/mL) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
ABCA9 polyclonal antibody (Cat # PAB11478) Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — ABCA9
Entrez GeneID
10350Protein Accession#
NP_525022.2Gene Name
ABCA9
Gene Alias
DKFZp686F2450, EST640918, MGC75415
Gene Description
ATP-binding cassette, sub-family A (ABC1), member 9
Gene Ontology
HyperlinkGene Summary
This gene is a member of the superfamily of ATP-binding cassette (ABC) transporters and the encoded protein contains two transmembrane domains and two nucleotide binding folds. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White). This gene is a member of the ABC1 subfamily and is clustered with four other ABC1 family members on chromosome 17q24. Transcriptional expression of this gene is induced during monocyte differentiation into macrophages and is suppressed by cholesterol import. [provided by RefSeq
Other Designations
ATP-binding cassette A9|ATP-binding cassette, sub-family A, member 9
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Interactome
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Pathway
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Publication Reference
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Molecular structure of a novel cholesterol-responsive A subclass ABC transporter, ABCA9.
Piehler A, Kaminski WE, Wenzel JJ, Langmann T, Schmitz G.
Biochemical and Biophysical Research Communications 2002 Jul; 295(2):408.
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Molecular structure of a novel cholesterol-responsive A subclass ABC transporter, ABCA9.
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