MBNL1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MBNL1.
Immunogen
A synthetic peptide corresponding to human MBNL1.
Sequence
C-SAEHLTSHKYVTQ
Host
Goat
Theoretical MW (kDa)
41, 39.8, 41.8, 33.1
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:4000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
MBNL1 polyclonal antibody (Cat # PAB11459) (1 ug/mL) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MBNL1
Entrez GeneID
4154Protein Accession#
NP_066368.2;NP_997175.1;NP_997176.1;NP_997177.1;NP_997178.1;NP_997179.1;NP_997180.1Gene Name
MBNL1
Gene Alias
DKFZp686P06174, EXP, EXP35, EXP40, EXP42, KIAA0428, MBNL
Gene Description
muscleblind-like (Drosophila)
Omim ID
606516Gene Ontology
HyperlinkOther Designations
muscleblind-like 1
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Interactome
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Publication Reference
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MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T.
Warf MB, Berglund JA.
RNA 2007 Dec; 13(12):2238.
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MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T.
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