ALOX15 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ALOX15.
Immunogen
A synthetic peptide corresponding to human ALOX15.
Sequence
C-HYKTDVAVKDDPE
Host
Goat
Theoretical MW (kDa)
74.8
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.2-0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
ALOX15 polyclonal antibody (Cat # PAB11445) (0.2 ug/mL) staining of HeLa nuclear extract (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ALOX15
Entrez GeneID
246Protein Accession#
NP_001131.3Gene Name
ALOX15
Gene Alias
15-LOX-1
Gene Description
arachidonate 15-lipoxygenase
Omim ID
152392Gene Ontology
HyperlinkOther Designations
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Interactome
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Pathway
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Disease
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Publication Reference
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Nonobese diabetic (NOD) mice congenic for a targeted deletion of 12/15-lipoxygenase are protected from autoimmune diabetes.
McDuffie M, Maybee NA, Keller SR, Stevens BK, Garmey JC, Morris MA, Kropf E, Rival C, Ma K, Carter JD, Tersey SA, Nunemaker CS, Nadler JL.
Diabetes 2008 Jan; 57(1):199.
Application:WB-Ce, Mouse, Mouse macrophages.
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Nonobese diabetic (NOD) mice congenic for a targeted deletion of 12/15-lipoxygenase are protected from autoimmune diabetes.
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