KRT13 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
KRT13 polyclonal antibody (Cat # PAB11444) (0.1 ug/mL) staining of human lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
KRT13 polyclonal antibody (Cat # PAB11444) (2.5 ug/mL) staining of paraffin embedded human tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of KRT13.
Immunogen
A synthetic peptide corresponding to human KRT13.
Sequence
C-TSNASGRRTSDVRRP
Host
Goat
Theoretical MW (kDa)
49.6, 45.8
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Western Blot (0.1-0.3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
KRT13 polyclonal antibody (Cat # PAB11444) (0.1 ug/mL) staining of human lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
KRT13 polyclonal antibody (Cat # PAB11444) (2.5 ug/mL) staining of paraffin embedded human tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — KRT13
Entrez GeneID
3860Protein Accession#
NP_705694.2Gene Name
KRT13
Gene Alias
CK13, K13, MGC161462, MGC3781
Gene Description
keratin 13
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been described. [provided by RefSeq
Other Designations
cytokeratin 13|keratin, type I cytoskeletal 13
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Interactome
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Publication Reference
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Kaposi's sarcoma-associated herpesvirus (KSHV) oncoprotein K13 bypasses TRAFs and directly interacts with the IkappaB kinase complex to selectively activate NF-kappaB without JNK activation.
Matta H, Mazzacurati L, Schamus S, Yang T, Sun Q, Chaudhary PM.
The Journal of Biological Chemistry 2007 Aug; 282(34):24858.
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Identification and molecular analysis of cable pilus biosynthesis genes in Burkholderia cepacia.
Umadevi S Sajjan, Hong Xie, Matthew D Lefebre, Miguel A Valvano, Janet F Forstner.
Microbiology (Reading, England) 2003 Apr; 149(Pt 4):961.
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Kaposi's sarcoma-associated herpesvirus (KSHV) oncoprotein K13 bypasses TRAFs and directly interacts with the IkappaB kinase complex to selectively activate NF-kappaB without JNK activation.
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