SIGLEC8 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SIGLEC8.
Immunogen
A synthetic peptide corresponding to human SIGLEC8.
Sequence
C-SYKSQLNYKTKQ
Host
Goat
Theoretical MW (kDa)
54
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
SIGLEC8 polyclonal antibody (Cat # PAB11442) (0.3 ug/mL) staining of MOLT 4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SIGLEC8
Entrez GeneID
27181Protein Accession#
NP_055257.2Gene Name
SIGLEC8
Gene Alias
MGC59785, SAF2, SIGLEC-8, SIGLEC8L
Gene Description
sialic acid binding Ig-like lectin 8
Omim ID
605639Gene Ontology
HyperlinkOther Designations
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Disease
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Publication Reference
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Inhibition of FcepsilonRI-dependent mediator release and calcium flux from human mast cells by sialic acid-binding immunoglobulin-like lectin 8 engagement.
Yokoi H, Choi OH, Hubbard W, Lee HS, Canning BJ, Lee HH, Ryu SD, von Gunten S, Bickel CA, Hudson SA, Macglashan DW Jr, Bochner BS.
The Journal of Allergy and Clinical Immunology 2008 Feb; 121(2):499.
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Ligation of Siglec-8: a selective mechanism for induction of human eosinophil apoptosis.
Nutku E, Aizawa H, Hudson SA, Bochner BS.
Blood 2003 Jun; 101(12):5014.
Application:Flow Cyt, WB, Human, HEK 293T cells, Human eosinophil.
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Inhibition of FcepsilonRI-dependent mediator release and calcium flux from human mast cells by sialic acid-binding immunoglobulin-like lectin 8 engagement.
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