APOL1 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
APOL1 polyclonal antibody (Cat # PAB11435) (0.1 ug/mL) staining of human placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of APOL1.
Immunogen
A synthetic peptide corresponding to C-terminus of human APOL1.
Sequence
C-NNNYKILQADQE
Host
Goat
Theoretical MW (kDa)
44, 45.9
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_003652.2; NP_663318.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
APOL1 polyclonal antibody (Cat # PAB11435) (0.1 ug/mL) staining of human placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — APOL1
Entrez GeneID
8542Protein Accession#
NP_003652.2;NP_663318.1Gene Name
APOL1
Gene Alias
APO-L, APOL, APOL-I
Gene Description
apolipoprotein L, 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000028705|apolipoprotein L-I|apolipoprotein L1
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Interactome
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Disease
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Publication Reference
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Apolipoprotein L-I promotes trypanosome lysis by forming pores in lysosomal membranes.
Perez-Morga D, Vanhollebeke B, Paturiaux-Hanocq F, Nolan DP, Lins L, Homble F, Vanhamme L, Tebabi P, Pays A, Poelvoorde P, Jacquet A, Brasseur R, Pays E.
Science 2005 Jul; 309(5733):469.
Application：IF, Bacteria, Trypanosome.
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Apolipoprotein L-I promotes trypanosome lysis by forming pores in lysosomal membranes.
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