LTF polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
LTF polyclonal antibody (Cat # PAB11431) (0.05 ug/mL) staining of human peripheral blood mononucleocyte (PBMCs) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
LTF polyclonal antibody (Cat # PAB11431) (2 ug/mL) staining of paraffin embedded human blood vessel. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Sandwich ELISA
LTF polyclonal antibody (Cat # PAB11431) (5 ug/mL) as the reporter with the capture rabbit antibody (5 ug/mL).
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of LTF.
Immunogen
A synthetic peptide corresponding to human LTF.
Sequence
C-ENYKSQQSSDPDP
Host
Goat
Theoretical MW (kDa)
78.2
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (0.05-0.2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
LTF polyclonal antibody (Cat # PAB11431) (0.05 ug/mL) staining of human peripheral blood mononucleocyte (PBMCs) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
LTF polyclonal antibody (Cat # PAB11431) (2 ug/mL) staining of paraffin embedded human blood vessel. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA
LTF polyclonal antibody (Cat # PAB11431) (5 ug/mL) as the reporter with the capture rabbit antibody (5 ug/mL). -
Gene Info — LTF
Entrez GeneID
4057Protein Accession#
NP_002334.2Gene Name
LTF
Gene Alias
GIG12, HLF2, LF
Gene Description
lactotransferrin
Omim ID
150210Gene Ontology
HyperlinkGene Summary
This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. [provided by RefSeq
Other Designations
growth-inhibiting protein 12|lactoferrin|neutrophil lactoferrin|talalactoferrin
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Interactome
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Disease
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Publication Reference
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Lactoferrin acts as an alarmin to promote the recruitment and activation of APCs and antigen-specific immune responses.
de la Rosa G, Yang D, Tewary P, Varadhachary A, Oppenheim JJ.
Journal of Immunology 2008 May; 180(10):6868.
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Lactoferrin acts as an alarmin to promote the recruitment and activation of APCs and antigen-specific immune responses.
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