ERP29 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
ERP29 polyclonal antibody (Cat # PAB11430) (0.1 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ERP29.
Immunogen
A synthetic peptide corresponding to human ERP29.
Sequence
C-EKNKMSDGKKEELQ
Host
Goat
Theoretical MW (kDa)
29, 5.48
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
ERP29 polyclonal antibody (Cat # PAB11430) (0.1 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ERP29
Entrez GeneID
10961Protein Accession#
NP_006808.1Gene Name
ERP29
Gene Alias
C12orf8, ERp28, ERp31, PDI-DB
Gene Description
endoplasmic reticulum protein 29
Omim ID
602287Gene Ontology
HyperlinkGene Summary
This gene encodes a reticuloplasmin, a protein which resides in the lumen of the endoplasmic reticulum (ER). The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
endoplasmic reticulum lumenal protein ERp28
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Interactome
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Publication Reference
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A chaperone-activated nonenveloped virus perforates the physiologically relevant endoplasmic reticulum membrane.
Rainey-Barger EK, Magnuson B, Tsai B.
Journal of Virology 2007 Sep; 81(23):12996.
Application：WB, Dog, Dog pancreatic microsomes.
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A chaperone-activated nonenveloped virus perforates the physiologically relevant endoplasmic reticulum membrane.
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