COG7 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
COG7 polyclonal antibody (Cat # PAB11426) (0.03 ug/mL) staining of MOLT 4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of COG7.
Immunogen
A synthetic peptide corresponding to human COG7.
Sequence
C-KTRPEDYRQVSK
Host
Goat
Theoretical MW (kDa)
86.3
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.03-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
COG7 polyclonal antibody (Cat # PAB11426) (0.03 ug/mL) staining of MOLT 4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — COG7
Entrez GeneID
91949Protein Accession#
NP_705831.1Gene Name
COG7
Gene Alias
CDG2E
Gene Description
component of oligomeric golgi complex 7
Gene Ontology
HyperlinkGene Summary
Multiprotein complexes are key determinants of Golgi apparatus structure and its capacity for intracellular transport and glycoprotein modification. Several complexes have been identified, including the Golgi transport complex (GTC), the LDLC complex, which is involved in glycosylation reactions, and the SEC34 complex, which is involved in vesicular transport. These 3 complexes are identical and have been termed the conserved oligomeric Golgi (COG) complex, which includes COG7 (Ungar et al., 2002 [PubMed 11980916]).[supplied by OMIM
Other Designations
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Interactome
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Publication Reference
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Genetic analysis of the subunit organization and function of the conserved oligomeric golgi (COG) complex: studies of COG5- and COG7-deficient mammalian cells.
Oka T, Vasile E, Penman M, Novina CD, Dykxhoorn DM, Ungar D, Hughson FM, Krieger M.
The Journal of Biological Chemistry 2005 Sep; 280(38):32736.
Application：WB-Tr, Human, Mouse, CHO, HeLa cells, Fibroblasts.
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Genetic analysis of the subunit organization and function of the conserved oligomeric golgi (COG) complex: studies of COG5- and COG7-deficient mammalian cells.
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