Dpm1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of Dpm1.
Immunogen
A synthetic peptide corresponding to mouse Dpm1.
Sequence
PQGRSSRQDKYS-C
Host
Goat
Theoretical MW (kDa)
29.2
Reactivity
Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Dpm1 polyclonal antibody (Cat # PAB11425) (0.05 ug/mL) staining of rat liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Dpm1
Entrez GeneID
13480Protein Accession#
NP_034202.1Gene Name
Dpm1
Gene Alias
AI118379, AI194292
Gene Description
dolichol-phosphate (beta-D) mannosyltransferase 1
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000017164
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Publication Reference
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Quantification of the Host Response Proteome after Herpes Simplex 1 Virus infection.
Berard AR, Coombs KM, Severini A.
Journal of Proteome Research 2015 May; 14(5):2121.
Application:WB, Human, HEK293 cells.
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DPM1, the catalytic subunit of dolichol-phosphate mannose synthase, is tethered to and stabilized on the endoplasmic reticulum membrane by DPM3.
Ashida H, Maeda Y, Kinoshita T.
The Journal of Biological Chemistry 2006 Jan; 281(2):896.
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Quantification of the Host Response Proteome after Herpes Simplex 1 Virus infection.
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