CLCA1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CLCA1.
Immunogen
A synthetic peptide corresponding to amino acids 872-884 of human CLCA1.
Sequence
PETPSPDETSAPC
Host
Goat
Theoretical MW (kDa)
100
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
CLCA1 polyclonal antibody (Cat # PAB11420) (0.3 ug/mL) staining of human lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CLCA1
Entrez GeneID
1179Protein Accession#
NP_001276.2Gene Name
CLCA1
Gene Alias
CACC, CACC1, CLCRG1, FLJ95147, GOB5
Gene Description
chloride channel regulator 1
Omim ID
603906Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the calcium sensitive chloride conductance protein family. To date, all members of this gene family map to the same region on chromosome 1p31-p22 and share a high degree of homology in size, sequence, and predicted structure, but differ significantly in their tissue distributions. The encoded protein is expressed as a precursor protein that is processed into two cell-surface-associated subunits, although the site at which the precursor is cleaved has not been precisely determined. The encoded protein may be involved in mediating calcium-activated chloride conductance in the intestine. [provided by RefSeq
Other Designations
CLCA family member 1, chloride channel regulator|OTTHUMP00000011907|calcium-activated chloride channel protein 1|calcium-dependent chloride channel-1|chloride channel, calcium activated, family member 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Oxidant stress stimulates Ca2+-activated chloride channels in the apical activated membrane of cultured nonciliated human nasal epithelial cells.
Jeulin C, Guadagnini R, Marano F.
American Journal of Physiology. Lung Cellular and Molecular Physiology 2005 Oct; 289(4):L636.
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Oxidant stress stimulates Ca2+-activated chloride channels in the apical activated membrane of cultured nonciliated human nasal epithelial cells.
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