Fto polyclonal antibody
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Specifications
Product Description
Goat polyclonal antibody raised against synthetic peptide of Fto.
Immunogen
A synthetic peptide corresponding to mouse Fto.
Sequence
QQKPDCRPYWEKDD
Host
Goat
Theoretical MW (kDa)
58
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Western Blot (0.03-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Fto polyclonal antibody (Cat # PAB11419) (0.03 ug/mL) staining of human pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Fto
Entrez GeneID
26383Protein Accession#
NP_036066.2Gene Name
Fto
Gene Alias
AW743446, mKIAA1752
Gene Description
fat mass and obesity associated
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000031320|fat mass and obesity-associated protein
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Publication Reference
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FTO modulates circadian rhythms and inhibits the CLOCK-BMAL1-induced transcription.
Wang CY, Shie SS, Hsieh IC, Tsai ML, Wen MS.
Biochemical and Biophysical Research Communications 2015 Aug; 464(3):826.
Application:IF, IP-WB, WB-Ce, WB-Ti, Mouse, Livers, MEF, NIH-3T3 cells.
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The obesity-associated FTO gene encodes a 2-oxoglutarate-dependent nucleic acid demethylase.
Gerken T, Girard CA, Tung YC, Webby CJ, Saudek V, Hewitson KS, Yeo GS, McDonough MA, Cunliffe S, McNeill LA, Galvanovskis J, Rorsman P, Robins P, Prieur X, Coll AP, Ma M, Jovanovic Z, Farooqi IS, Sedgwick B, Barroso I, Lindahl T, Ponting CP, Ashcroft FM, O'Rahilly S, Schofield CJ.
Science 2007 Nov; 318(5855):1469.
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FTO modulates circadian rhythms and inhibits the CLOCK-BMAL1-induced transcription.
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