SPHK1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
SPHK1 polyclonal antibody (Cat # PAB11412) (2 ug/mL) staining of human peripheral blood mononucleocyte (PBMCs) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SPHK1.
Immunogen
A synthetic peptide corresponding to human SPHK1.
Sequence
C-DVDLESEKYRRLGE
Host
Goat
Theoretical MW (kDa)
43.9, 51.1
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (2-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
SPHK1 polyclonal antibody (Cat # PAB11412) (2 ug/mL) staining of human peripheral blood mononucleocyte (PBMCs) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — SPHK1
Entrez GeneID
8877Protein Accession#
NP_068807.2;NP_892010.1Gene Name
SPHK1
Gene Alias
SPHK
Gene Description
sphingosine kinase 1
Omim ID
603730Gene Ontology
HyperlinkGene Summary
Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM
Other Designations
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Interactome
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Pathway
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Publication Reference
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Globular adiponectin induces adhesion molecule expression through the sphingosine kinase pathway in vascular endothelial cells.
Kase H, Hattori Y, Jojima T, Okayasu T, Tomizawa A, Suzuki K, Banba N, Monden T, Satoh H, Akimoto K, Kasai K.
Life Sciences 2007 Aug; 81(11):939.
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Globular adiponectin induces adhesion molecule expression through the sphingosine kinase pathway in vascular endothelial cells.
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