Western blot analysis is shown using swi6 polyclonal antibody (Cat # PAB11378) to detect endogenous protein present in S.pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicatesa band of ~43 KDa corresponding to S.pombe Swi6 protein. Approx. ~35 ug of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 um nitrocellulose. The blot was incubated with a 1:1,700 dilution of the antibody at room temperature for 2 hrs.