Rabbit polyclonal antibody raised against full length recombinant SARS N.
Immunogen:
Recombinant protein corresponding to full length SARS N (Nucleocapsid protein).
Host:
Rabbit
Reactivity:
SARS-CoV
Specificity:
This protein A purified antibody is directed against SARS Coronavirus Nucleocapsid (N) protein.
Form:
Lyophilized
Purification:
The product was purified from monospecific antiserum by protein A affinity purification.
Recommend Usage:
ELISA (1:10000-1:50000) Flow Cytometry Immunofluorescence Immunohistochemistry (1:100-1:6000) Western Blot (1:2000-1:10000) The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide).
Storage Instruction:
Store at 4°C on dry atmosphere prior to restoration. After reconstitution with 100 uL of deionized water (or equivalent), aliquot and store at -20°C or lower. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot using SARS-CoV N polyclonal antibody (Cat # PAB11373) shows detection of a 46- KDa band corresponding to the protein. Approx. 100 ng of protein was loaded for SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:5,000 dilution of the antibody at room temperature for 1 h.