Rabbit polyclonal antibody raised against full length recombinant SARS-CoV nonstructural protein 8 (nsp8).
Immunogen:
Recombinant protein corresponding to full length SARS-CoV nsp8.
Host:
Rabbit
Reactivity:
SARS-CoV
Specificity:
This antibody is directed against SARS-Coronavirus nsp8 protein.
Form:
Liquid
Purity:
The product is neat antiserum.
Recommend Usage:
Immunoelectron Microscopy (1:100) Immunofluorescence (1:300) Immunoprecipitation (1:60) Western Blot (1:1000) The optimal working dilution should be determined by the end user.
Storage Buffer:
In antiserum (0.01% sodium azide).
Storage Instruction:
Store at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Aliquot to avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunoprecipitation followed by western blotting using SARS-CoV nsp8 polyclonal antibody (Cat # PAB11372) shows a predominant band at 21.8 KDa corresponding to full length SARS protein. Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.
Immunofluorescence
Immunofluorescence Microscopy using SARS-CoV nsp8 polyclonal antibody (Cat # PAB11372) 6 hrs post infection of Vero-E6 cells. Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.