Western blot using ULP1 polyclonal antibody (Cat # PAB11365) shows detection of a truncated ULP1 fusion protein (arrowhead). Increasing concentrations of yeast ULP1 were run on a SDS-PAGE, transferred onto nitrocellulose, and blocked for 1 hour with 5% non-fat dry milk in TTBS, and probed overnight at 4°C with a 1:1000 dilution of anti-yULP1 antibody in 5% non-fat dry milk inTTBS. Detection occurred using a 1:1,000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 3-sec exposure time.
Ubl (ubiquitin-like protein)-specific protease that cleaves Smt3p protein conjugates; specifically required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase