ULP1 polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot using ULP1 polyclonal antibody (Cat # PAB11365) shows detection of a truncated ULP1 fusion protein (arrowhead).
Increasing concentrations of yeast ULP1 were run on a SDS-PAGE, transferred onto nitrocellulose, and blocked for 1 hour with 5% non-fat dry milk in TTBS, and probed overnight at 4°C with a 1:1000 dilution of anti-yULP1 antibody in 5% non-fat dry milk inTTBS.
Detection occurred using a 1:1,000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature.
A chemiluminescence system was used for signal detection (Roche) using a 3-sec exposure time.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant ULP1.
Immunogen
Recombinant protein corresponding to Saccharomyces cerevisiae ULP1.
Host
Rabbit
Reactivity
Yeast
Form
Lyophilized
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:4000-1:20000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 100 uL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot using ULP1 polyclonal antibody (Cat # PAB11365) shows detection of a truncated ULP1 fusion protein (arrowhead).
Increasing concentrations of yeast ULP1 were run on a SDS-PAGE, transferred onto nitrocellulose, and blocked for 1 hour with 5% non-fat dry milk in TTBS, and probed overnight at 4°C with a 1:1000 dilution of anti-yULP1 antibody in 5% non-fat dry milk inTTBS.
Detection occurred using a 1:1,000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature.
A chemiluminescence system was used for signal detection (Roche) using a 3-sec exposure time.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — ULP1
Entrez GeneID
856087Protein Accession#
NP_015305;Q02724Gene Name
ULP1
Gene Alias
NIB1
Gene Description
Ubl (ubiquitin-like protein)-specific protease that cleaves Smt3p protein conjugates; specifically required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase
Gene Ontology
HyperlinkOther Designations
Ulp1p
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Publication Reference
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The Ulp1 SUMO isopeptidase: distinct domains required for viability, nuclear envelope localization, and substrate specificity.
Li SJ, Hochstrasser M.
The Journal of Cell Biology 2003 Mar; 160(7):1069.
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A lack of SUMO conjugation affects cNLS-dependent nuclear protein import in yeast.
Stade K, Vogel F, Schwienhorst I, Meusser B, Volkwein C, Nentwig B, Dohmen RJ, Sommer T.
The Journal of Biological Chemistry 2002 Oct; 277(51):49554.
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Ulp1-SUMO crystal structure and genetic analysis reveal conserved interactions and a regulatory element essential for cell growth in yeast.
Mossessova E, Lima CD.
Molecular Cell 2000 May; 5(5):865.
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The Ulp1 SUMO isopeptidase: distinct domains required for viability, nuclear envelope localization, and substrate specificity.
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