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Last updated: 2016/11/27
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ULP1 polyclonal antibody

  • Catalog # : PAB11365
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against recombinant ULP1.
  • Immunogen:
  • Recombinant protein corresponding to Saccharomyces cerevisiae ULP1.
  • Host:
  • Rabbit
  • Reactivity:
  • Yeast
  • Form:
  • Lyophilized
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA (1:4000-1:20000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C on dry atmosphere.
    After reconstitution with 100 uL of deionized water, store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot using ULP1 polyclonal antibody (Cat # PAB11365) shows detection of a truncated ULP1 fusion protein (arrowhead).
    Increasing concentrations of yeast ULP1 were run on a SDS-PAGE, transferred onto nitrocellulose, and blocked for 1 hour with 5% non-fat dry milk in TTBS, and probed overnight at 4°C with a 1:1000 dilution of anti-yULP1 antibody in 5% non-fat dry milk inTTBS.
    Detection occurred using a 1:1,000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature.
    A chemiluminescence system was used for signal detection (Roche) using a 3-sec exposure time.
  • Immunofluorescence
  • ELISA
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunofluorescence
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_015305;Q02724
  • Gene Name:
  • ULP1
  • Gene Alias:
  • NIB1
  • Gene Description:
  • Ubl (ubiquitin-like protein)-specific protease that cleaves Smt3p protein conjugates; specifically required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase
  • Other Designations:
  • Ulp1p
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