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Last updated: 2016/12/4
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RFA2 polyclonal antibody

  • Catalog # : PAB11364
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of RFA2.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 115-129 of Saccharomyces cerevisiae RFA2.
  • Sequence:
  • SSGKGYGSQVAQQFE
  • Host:
  • Rabbit
  • Reactivity:
  • Yeast
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:1000-1:3000)
    Western Blot (1:100-1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blotting against RFA2 containing cell lysates. Lysates from Saccharomyces sp. :
    Lane 1, wild type.
    Lane 2, constitutional unphosphorylated.
    Lane 3, constitutional phosphorylated.
    Lane 4 induced phosphorylation.
    Blot incubated for 1 hr at RT with affinity purified Rabbit anti RFA2 pan reactive at 1.0 ug/mL followed by 1 : 5000 dilution of IRDye800 Sheep anti Rabbit IgG for 1 hr at RT.
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blotting against RFA2 containing cell lysates. Lysates from Saccharomyces sp. :
    Lane 1, wild type.
    Lane 2, constitutional unphosphorylated.
    Lane 3, constitutional phosphorylated.
    Lane 4 induced phosphorylation.
    Blot incubated for 1 hr at RT with affinity purified Rabbit anti RFA2 pan reactive at 1.0 ug/mL followed by 1 : 5000 dilution of IRDye800 Sheep anti Rabbit IgG for 1 hr at RT.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Gene Name:
  • RFA2
  • Gene Alias:
  • BUF1,RPA2
  • Gene Description:
  • Rfa2p
  • Other Designations:
  • Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination
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