RFA2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blotting against RFA2 containing cell lysates. Lysates from Saccharomyces sp. :
Lane 1, wild type.
Lane 2, constitutional unphosphorylated.
Lane 3, constitutional phosphorylated.
Lane 4 induced phosphorylation.
Blot incubated for 1 hr at RT with affinity purified Rabbit anti RFA2 pan reactive at 1.0 ug/mL followed by 1 : 5000 dilution of IRDye800 Sheep anti Rabbit IgG for 1 hr at RT.
Western Blot (Cell lysate)
Western blotting against RFA2 containing cell lysates. Lysates from Saccharomyces sp. :
Lane 1, wild type.
Lane 2, constitutional unphosphorylated.
Lane 3, constitutional phosphorylated.
Lane 4 induced phosphorylation.
Blot incubated for 1 hr at RT with affinity purified Rabbit anti RFA2 pan reactive at 1.0 ug/mL followed by 1 : 5000 dilution of IRDye800 Sheep anti Rabbit IgG for 1 hr at RT.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RFA2.
Immunogen
A synthetic peptide corresponding to amino acids 115-129 of Saccharomyces cerevisiae RFA2.
Sequence
SSGKGYGSQVAQQFE
Host
Rabbit
Reactivity
Yeast
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:1000-1:3000)
Western Blot (1:100-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blotting against RFA2 containing cell lysates. Lysates from Saccharomyces sp. :
Lane 1, wild type.
Lane 2, constitutional unphosphorylated.
Lane 3, constitutional phosphorylated.
Lane 4 induced phosphorylation.
Blot incubated for 1 hr at RT with affinity purified Rabbit anti RFA2 pan reactive at 1.0 ug/mL followed by 1 : 5000 dilution of IRDye800 Sheep anti Rabbit IgG for 1 hr at RT.Western Blot (Cell lysate)
Western blotting against RFA2 containing cell lysates. Lysates from Saccharomyces sp. :
Lane 1, wild type.
Lane 2, constitutional unphosphorylated.
Lane 3, constitutional phosphorylated.
Lane 4 induced phosphorylation.
Blot incubated for 1 hr at RT with affinity purified Rabbit anti RFA2 pan reactive at 1.0 ug/mL followed by 1 : 5000 dilution of IRDye800 Sheep anti Rabbit IgG for 1 hr at RT.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — RFA2
Entrez GeneID
855404Gene Name
RFA2
Gene Alias
BUF1, RPA2
Gene Description
Rfa2p
Gene Ontology
HyperlinkGene Summary
which is a highly conserved single-stranded DNA binding protein involved in DNA replication
Other Designations
Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination
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