RAD9 polyclonal antibody
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More Files
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Western Blot (Recombinant protein)
Affinity purified antibody to yeast RAD9 (1125-1139 pan reactive) was used at a 1:200 dilution incubated 8 h at room temperature to detect RAD9 by Western blot.
Lanes were loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae RAD9 (aa 991-1309 ~60 kDa) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast RAD9, Lane 2 - in vitro phosphorylated wild type yeast RAD9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant RAD9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant.
Phosphorylation of RAD9 was by treatment with ATP and Rad53 kinase.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RAD9.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 1125-1139 residues surrounding S1129 of Saccharomyces cerevisiae RAD9.
Host
Rabbit
Reactivity
Yeast
Specificity
This pan reactive polyclonal antibody reacts equally with both phosphorylated and non-phosphorylated yeast Rad9 at S1129. No reactivity is expected against human and mouse homologs.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Sandwich ELISA (1:5000)
Western Blot (1:100-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Affinity purified antibody to yeast RAD9 (1125-1139 pan reactive) was used at a 1:200 dilution incubated 8 h at room temperature to detect RAD9 by Western blot.
Lanes were loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae RAD9 (aa 991-1309 ~60 kDa) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast RAD9, Lane 2 - in vitro phosphorylated wild type yeast RAD9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant RAD9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant.
Phosphorylation of RAD9 was by treatment with ATP and Rad53 kinase.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RAD9
Entrez GeneID
851803Protein Accession#
Locus:11q13.1-q13.2;OMIM603761;GDB5592334;SwissProtQ99638Gene Name
RAD9
Gene Alias
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Gene Description
DNA damage-dependent checkpoint protein, required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; potential Cdc28p substrate
Gene Ontology
HyperlinkGene Summary
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Other Designations
Rad9p
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Publication Reference
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Rad9 phosphorylation sites couple Rad53 to the Saccharomyces cerevisiae DNA damage checkpoint.
Schwartz MF, Duong JK, Sun Z, Morrow JS, Pradhan D, Stern DF.
Molecular Cell 2002 May; 9(5):1055.
Application：IP, WB, Yeast, Saccharomyces cerevisiae.
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Schizosaccharomyces pombe Rad9 contains a BH3-like region and interacts with the anti-apoptotic protein Bcl-2.
Komatsu K, Hopkins KM, Lieberman HB, Wang H.
FEBS Letters 2000 Sep; 481(2):122.
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Rad53 FHA domain associated with phosphorylated Rad9 in the DNA damage checkpoint.
Sun Z, Hsiao J, Fay DS, Stern DF.
Science 1998 Jul; 281(5374):272.
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Rad9 phosphorylation sites couple Rad53 to the Saccharomyces cerevisiae DNA damage checkpoint.
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