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Last updated: 2016/11/27
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RAD9 polyclonal antibody

  • Catalog # : PAB11362
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of RAD9.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to amino acids 1125-1139 residues surrounding S1129 of Saccharomyces cerevisiae RAD9.
  • Host:
  • Rabbit
  • Reactivity:
  • Yeast
  • Specificity:
  • This pan reactive polyclonal antibody reacts equally with both phosphorylated and non-phosphorylated yeast Rad9 at S1129. No reactivity is expected against human and mouse homologs.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Sandwich ELISA (1:5000)
    Western Blot (1:100-1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Affinity purified antibody to yeast RAD9 (1125-1139 pan reactive) was used at a 1:200 dilution incubated 8 h at room temperature to detect RAD9 by Western blot.
    Lanes were loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae RAD9 (aa 991-1309 ~60 kDa) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast RAD9, Lane 2 - in vitro phosphorylated wild type yeast RAD9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant RAD9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant.
    Phosphorylation of RAD9 was by treatment with ATP and Rad53 kinase.
  • ELISA
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • Locus:11q13.1-q13.2;OMIM603761;GDB5592334;SwissProtQ99638
  • Gene Name:
  • RAD9
  • Gene Alias:
  • -
  • Gene Description:
  • DNA damage-dependent checkpoint protein, required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; potential Cdc28p substrate
  • Other Designations:
  • Rad9p
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