HUB1 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Recombinant protein)
Immunoblot of HUB1 fusion protein.
HUB1 polyclonal antibody (Cat # PAB11361) generated by immunization with recombinant yeast HUB1 was tested by immunoblot against yeast lysates expressing the HUB1-GFP fusion protein and other UBL fusion proteins.
All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL.
Panel A shows total protein staining using ponceau.
Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of anti-GFP followed by reaction with a 1:15,000 dilution of HRP Donkey-a-Goat IgG MX.
Panel C shows specific reaction with Hub1 using a 1:500 dilution of HUB1 polyclonal antibody (Cat # PAB11361) (Yeast) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX.
All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4°C.
Yeast lysate proteins were separated by SDS-PAGE using a 15% gel. This data indicates that anti-HUB1 is highly specific and does not cross react with other UBLs.
A chemiluminescence system was used for signal detection (Roche) .
Data contributed by M. Malakhov, www.lifesensors.com, personal communication.
-
Specification
Product Description
Rabbit polyclonal antibody raised against recombinant HUB1.
Immunogen
Recombinant protein corresponding to Saccharomyces cerevisiae HUB1.
Host
Rabbit
Reactivity
Yeast
Form
Lyophilized
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:1000-1:5000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot after reconstitution to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Recombinant protein)
Immunoblot of HUB1 fusion protein.
HUB1 polyclonal antibody (Cat # PAB11361) generated by immunization with recombinant yeast HUB1 was tested by immunoblot against yeast lysates expressing the HUB1-GFP fusion protein and other UBL fusion proteins.
All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL.
Panel A shows total protein staining using ponceau.
Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of anti-GFP followed by reaction with a 1:15,000 dilution of HRP Donkey-a-Goat IgG MX.
Panel C shows specific reaction with Hub1 using a 1:500 dilution of HUB1 polyclonal antibody (Cat # PAB11361) (Yeast) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX.
All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4°C.
Yeast lysate proteins were separated by SDS-PAGE using a 15% gel. This data indicates that anti-HUB1 is highly specific and does not cross react with other UBLs.
A chemiluminescence system was used for signal detection (Roche) .
Data contributed by M. Malakhov, www.lifesensors.com, personal communication.Enzyme-linked Immunoabsorbent Assay
-
Gene Info — HUB1
Entrez GeneID
855767Protein Accession#
Q6Q546Gene Name
HUB1
Gene Alias
-
Gene Description
Hub1p
Gene Ontology
HyperlinkGene Summary
may function in modification of Sph1p and Hbt1p
Other Designations
Ubiquitin-like protein modifier, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear
-
Publication Reference
-
Structural analysis of UBL5, a novel ubiquitin-like modifier.
McNally T, Huang Q, Janis RS, Liu Z, Olejniczak ET, Reilly RM.
Protein Science 2003 Jul; 12(7):1562.
-
Role of a ubiquitin-like modification in polarized morphogenesis.
Dittmar GA, Wilkinson CR, Jedrzejewski PT, Finley D.
Science 2002 Mar; 295(5564):2442.
-
Ubiquitin and its kin: how close are the family ties?
Jentsch S, Pyrowolakis G.
Trends in Cell Biology 2000 Aug; 10(8):335.
-
Structural analysis of UBL5, a novel ubiquitin-like modifier.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com