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Last updated: 2018/1/21
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CHK1 polyclonal antibody

  • Catalog # : PAB11360
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of CHK1.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 312-327 of Saccharomyces cerevisiae CHK1.
  • Host:
  • Rabbit
  • Reactivity:
  • Yeast
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:2000-1:10000)
    Western Blot (1:200-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using CHK1 polyclonal antibody (Cat # PAB11360) shows detection of bands corresponding to CHK1 in Saccharomyces cerevisiae lysates.
    Two strains of S.cerevisiae were tested. Lane 1 shows a predominant band at ~60 KDa. Lane 2 shows a predominant band at ~38 KDa.
    Specific band staining is blocked when antibody is preincubated for 45 min at room temperature with 50 ug of peptide immunogen (lanes 3 and 4 respectively).
    Lysates were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.
    After blocking, the membrane was probed for 2 h at room temperature with the primary antibody diluted to 1:750 in blocking buffer diluted 1:5 in PBS.
    The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 minat room temperature (800 nm channel, green).
    Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
    IRDye™800 fluorescence image was c
  • Immunoprecipitation
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunoprecipitation
  • Gene Information
  • Protein Accession#:
  • P38147;NP_009833
  • Gene Name:
  • CHK1
  • Gene Alias:
  • -
  • Gene Description:
  • Chk1p
  • Other Designations:
  • Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase
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