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Last updated: 2016/10/23
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ATG8 polyclonal antibody

  • Catalog # : PAB11359
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against recombinant ATG8.
  • Immunogen:
  • Recombinant protein corresponding to Saccharomyces cerevisiae ATG8.
  • Host:
  • Rabbit
  • Reactivity:
  • Yeast
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA (1:20000-1:100000)
    Western Blot (1:4000-1:8000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Immunoblot of ATG8 fusion protein.
    ATG8 polyclonal antibody (Cat # PAB11359) generated by immunization with recombinant yeast ATG8 was tested by immunoblot with other anti-UBL antibodies against E.coli lysates expressing the ATG8-GFP fusion protein.
    All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL.
    Panel A shows total protein staining using ponceau.
    Panel B shows specific reaction with APG8 using a 1:4,000 and 1:8,000 dilution of ATG8 polyclonal antibody (Cat # PAB11359) (Yeast) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX.
    All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4°C. E.coli lysate proteins were separated by SDS-PAGE using a 15% gel.
    This data indicates that anti-ATG8 is highly specific and does not cross react with other UBLs. A chemilum
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Gene Information
  • Gene Name:
  • ATG8
  • Gene Alias:
  • APG8,AUT7,CVT5
  • Gene Description:
  • Atg8p
  • Other Designations:
  • Protein required for autophagy; modified by the serial action of Atg4p, Atg7p, and Atg3p, and conjugated at the C terminus with phosphatidylethanolamine, to become the form essential for generation of autophagosomes
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