Western blot using MER2 polyclonal antibody (Cat # PAB11356) shows detection of phosphorylated and unphosphorylated MER2 in wild type, phosphatase treated and mutant cells. Lane 1 contains MER2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated MER2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains MER2-S30A protein as a phosphorylation control. This antibody is reactive with both phosphor-ylated and unphosphorylated MER2 at the S30 position. The primary antibody was used at a 1:5,000 dilution. Personal Communication. MichaelLichten, NIH, CCR, Bethesda, MD.
Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation