RUB1 polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
RUB1 polyclonal antibody (Cat # PAB11355), generated by immunization with full-length, recombinant yeast RUB1, was tested by immunoblot against a yeast cell lysate.
A dilution of the antibody between 1:200 and 1:1,000 will show strong reactivity specifically with free RUB1 protein (indicated by arrow) and RUB1 conjugates.
In this blot, the antibody was used at a 1:500 dilution and was incubated overnight at 4°C in 5% non-fat dry milk in TTBS.
Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature.
A chemiluminescence system was used for signal detection (Roche).
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant RUB1.
Immunogen
Recombinant protein corresponding to full length Saccharomyces cerevisiae RUB1.
Host
Rabbit
Reactivity
Yeast
Form
Lyophilized
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:1000-1:5000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot after reconstitution to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
RUB1 polyclonal antibody (Cat # PAB11355), generated by immunization with full-length, recombinant yeast RUB1, was tested by immunoblot against a yeast cell lysate.
A dilution of the antibody between 1:200 and 1:1,000 will show strong reactivity specifically with free RUB1 protein (indicated by arrow) and RUB1 conjugates.
In this blot, the antibody was used at a 1:500 dilution and was incubated overnight at 4°C in 5% non-fat dry milk in TTBS.
Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature.
A chemiluminescence system was used for signal detection (Roche).Enzyme-linked Immunoabsorbent Assay
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Gene Info — RUB1
Entrez GeneID
851717Gene Name
RUB1
Gene Alias
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Gene Description
Rub1p
Gene Ontology
HyperlinkGene Summary
Rtt101p
Other Designations
Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme)
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Publication Reference
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AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response.
del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M.
The Plant Cell 2002 Feb; 14(2):421.
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CSN1 N-terminal-dependent activity is required for Arabidopsis development but not for Rub1/Nedd8 deconjugation of cullins: a structure-function study of CSN1 subunit of COP9 signalosome.
Wang X, Kang D, Feng S, Serino G, Schwechheimer C, Wei N.
Molecular Biology of the Cell 2002 Feb; 13(2):646.
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The Arabidopsis cullin AtCUL1 is modified by the ubiquitin-related protein RUB1.
del Pozo JC, Estelle M.
PNAS 1999 Dec; 96(26):15342.
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AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response.
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