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Last updated: 2017/6/18
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RUB1 polyclonal antibody

  • Catalog # : PAB11355
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against full length recombinant RUB1.
  • Immunogen:
  • Recombinant protein corresponding to full length Saccharomyces cerevisiae RUB1.
  • Host:
  • Rabbit
  • Reactivity:
  • Yeast
  • Form:
  • Lyophilized
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA (1:1000-1:5000)
    Western Blot (1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot after reconstitution to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • RUB1 polyclonal antibody (Cat # PAB11355), generated by immunization with full-length, recombinant yeast RUB1, was tested by immunoblot against a yeast cell lysate.
    A dilution of the antibody between 1:200 and 1:1,000 will show strong reactivity specifically with free RUB1 protein (indicated by arrow) and RUB1 conjugates.
    In this blot, the antibody was used at a 1:500 dilution and was incubated overnight at 4°C in 5% non-fat dry milk in TTBS.
    Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature.
    A chemiluminescence system was used for signal detection (Roche).
  • ELISA
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • RUB1
  • Gene Alias:
  • -
  • Gene Description:
  • Rub1p
  • Other Designations:
  • Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme)
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