Product Browser

Last updated: 2017/3/26

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

S100A1/S100B polyclonal antibody

  • Catalog # : PAB11341
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against full length recombinant S100A1/S100B.
  • Immunogen:
  • Recombinant protein corresponding to full length bovine S100A1/S100B.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Dog, Mouse, Rat
  • Specificity:
  • A 98% homology is noted for S100 alpha chain from primate sources. Mouse, rat and dog show 94% homology with the bovine S100 alpha sequence. Expect cross reactivity with S100 from most mammalian sources. Homologies for the S100 beta chain are similar.
  • Form:
  • Lyophilized
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA (1:5000-1:25000)
    Western Blot (1:500-1:3000)
    Immunohistochemistry (1:200-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot after reconstitution to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Western blot using S100A1/S100B polyclonal antibody (Cat # PAB11341) shows detection of a band ~11 KDa corresponding to bovine S100A1/S100B monomer (100 ng loaded, arrowhead lane 1).
    The antibody also detects S100A1/S100B from rat brain lysate (Lane 2). Approximately 35 ug of a rat brain whole cell lysate was separated by 16% SDS-PAGE and transferred onto nitrocellulose.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of S100A1/S100B polyclonal antibody (Cat # PAB11341) was used at a 1:500 dilution to detect S100A1/S100B by immunohistochemistry in human brain astrocyte tumor tissue.
    Tissue was formalin-fixed and paraffin embedded.
    Personal Communication,Alan Yen, Life Span Biosciences, Seattle, WA.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of S100A1/S100B polyclonal antibody (Cat # PAB11341) was used at a 1:500 dilution to detect S100A1/S100B.
    Dark nuclear staining is observed within basket cells located near the Purkinje cells in the mouse cerebellum.
    Personal Communication, Teri Johnson, Stowers Institute, Kansas City, MO.
  • ELISA
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • S100A1
  • Gene Alias:
  • MGC159706
  • Gene Description:
  • S100 calcium binding protein A1
  • Other Designations:
  • -
  • Gene Information
  • Gene Name:
  • S100B
  • Gene Alias:
  • MGC128275
  • Gene Description:
  • S100 calcium binding protein B
  • Other Designations:
  • S100 protein, beta polypeptide
  • RSS
  • YouTube
  • Linkedin
  • Facebook