Rabbit polyclonal antibody raised against full length recombinant S100A1/S100B.
Recombinant protein corresponding to full length bovine S100A1/S100B.
Bovine, Dog, Mouse, Rat
A 98% homology is noted for S100 alpha chain from primate sources. Mouse, rat and dog show 94% homology with the bovine S100 alpha sequence. Expect cross reactivity with S100 from most mammalian sources. Homologies for the S100 beta chain are similar.
Quality Control Testing:
Antibody Reactive Against Recombinant Protein.
ELISA (1:5000-1:25000) Western Blot (1:500-1:3000) Immunohistochemistry (1:200-1:2000) The optimal working dilution should be determined by the end user.
Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot after reconstitution to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot using S100A1/S100B polyclonal antibody (Cat # PAB11341) shows detection of a band ~11 KDa corresponding to bovine S100A1/S100B monomer (100 ng loaded, arrowhead lane 1). The antibody also detects S100A1/S100B from rat brain lysate (Lane 2). Approximately 35 ug of a rat brain whole cell lysate was separated by 16% SDS-PAGE and transferred onto nitrocellulose.
Immunohistochemistry of S100A1/S100B polyclonal antibody (Cat # PAB11341) was used at a 1:500 dilution to detect S100A1/S100B by immunohistochemistry in human brain astrocyte tumor tissue. Tissue was formalin-fixed and paraffin embedded. Personal Communication,Alan Yen, Life Span Biosciences, Seattle, WA.
Immunohistochemistry of S100A1/S100B polyclonal antibody (Cat # PAB11341) was used at a 1:500 dilution to detect S100A1/S100B. Dark nuclear staining is observed within basket cells located near the Purkinje cells in the mouse cerebellum. Personal Communication, Teri Johnson, Stowers Institute, Kansas City, MO.