Zic2 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot using Zic2 polyclonal antibody (Cat # PAB11336) shows detection of a band ~55 KDa (arrowhead) corresponding to Zic2 in lysates from (lane1) mouse brain and (Lane 2) rat brain.
Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of Zic2 polyclonal antibody (Cat # PAB11336).
The upper band in the mouse brain lysate represents Zic2.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Zic2.
Immunogen
A synthetic peptide corresponding to amino acids 189-206 of mouse Zic2.
Host
Rabbit
Reactivity
Chicken, Chimpanzee, Dog, Frog, Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:50000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot using Zic2 polyclonal antibody (Cat # PAB11336) shows detection of a band ~55 KDa (arrowhead) corresponding to Zic2 in lysates from (lane1) mouse brain and (Lane 2) rat brain.
Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of Zic2 polyclonal antibody (Cat # PAB11336).
The upper band in the mouse brain lysate represents Zic2.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Zic2
Entrez GeneID
22772Protein Accession#
O95409;NP_009060Gene Name
Zic2
Gene Alias
HPE5, Ku
Gene Description
zinc finger protein of the cerebellum 2
Gene Ontology
HyperlinkGene Summary
Drosophila)|kumba|odd-paired homolog
Other Designations
GENA 29|OTTMUSP00000022013|Zic family member 2 (odd-paired homolog, Drosophila)|kumba|odd-paired homolog
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Publication Reference
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Molecular screening of SHH, ZIC2, SIX3, and TGIF genes in patients with features of holoprosencephaly spectrum: Mutation review and genotype-phenotype correlations.
Dubourg C, Lazaro L, Pasquier L, Bendavid C, Blayau M, Le Duff F, Durou MR, Odent S, David V.
Human Mutation 2004 Jul; 24(1):43.
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Zic2 patterns binocular vision by specifying the uncrossed retinal projection.
Herrera E, Brown L, Aruga J, Rachel RA, Dolen G, Mikoshiba K, Brown S, Mason CA.
Cell 2003 Sep; 114(5):545.
Application：IF, IHC, WB, Mouse, Mouse retina.
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Possible association of NTDs with a polyhistidine tract polymorphism in the ZIC2 gene.
Brown LY, Hodge SE, Johnson WG, Guy SG, Nye JS, Brown S.
American Journal of Medical Genetics 2002 Mar; 108(2):128.
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Molecular screening of SHH, ZIC2, SIX3, and TGIF genes in patients with features of holoprosencephaly spectrum: Mutation review and genotype-phenotype correlations.
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