Ntrk3 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot using Ntrk3 polyclonal antibody (Cat # PAB11334) to detect endogenous Ntrk3 in mouse cortex lysate (Lane 1). Lane 2 is Ntrk3 knock-out cortex lysate.
Cell extracts were resolved by electrophoresis and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:6,000 dilution.
Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.
Western Blot (Transfected lysate)
Western blot using Ntrk3 polyclonal antibody (Cat # PAB11334) to detect over-expressed Ntrk3 in HEK293 cells (Lane 2, arrowhead). Lane 1 is a non-transfected control.
Cell extracts were resolved by electrophoresis and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:3,000 dilution.
Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.
Immunoprecipitation
Mouse cortex lysate was immunoprecipitated with Ntrk3 polyclonal antibody (Cat # PAB11334) and further blotted with affinity purified anti-Ntrk3.
Lane 1 is wild-type cortex lysate, Lane 2 is Grasp knock-out cortex lysate, and Lane 3 is Ntrk3 knock-out cortex lysate.
The membrane was probed with the primary antibody at a 1:6,000 dilution.
Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Ntrk3.
Immunogen
A synthetic peptide corresponding to C-terminus of mouse Ntrk3.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:5000-1:20000)
Western Blot (1:1000-1:10000)
Immunoprecipitation (1 ug)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot using Ntrk3 polyclonal antibody (Cat # PAB11334) to detect endogenous Ntrk3 in mouse cortex lysate (Lane 1). Lane 2 is Ntrk3 knock-out cortex lysate.
Cell extracts were resolved by electrophoresis and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:6,000 dilution.
Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.Western Blot (Transfected lysate)
Western blot using Ntrk3 polyclonal antibody (Cat # PAB11334) to detect over-expressed Ntrk3 in HEK293 cells (Lane 2, arrowhead). Lane 1 is a non-transfected control.
Cell extracts were resolved by electrophoresis and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:3,000 dilution.
Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.Immunoprecipitation
Mouse cortex lysate was immunoprecipitated with Ntrk3 polyclonal antibody (Cat # PAB11334) and further blotted with affinity purified anti-Ntrk3.
Lane 1 is wild-type cortex lysate, Lane 2 is Grasp knock-out cortex lysate, and Lane 3 is Ntrk3 knock-out cortex lysate.
The membrane was probed with the primary antibody at a 1:6,000 dilution.
Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Ntrk3
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Publication Reference
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A kinase-deficient TrkC receptor isoform activates Arf6-Rac1 signaling through the scaffold protein tamalin.
Esteban PF, Yoon HY, Becker J, Dorsey SG, Caprari P, Palko ME, Coppola V, Saragovi HU, Randazzo PA, Tessarollo L.
Journal of Cellular Biology 2006 Apr; 173(2):291.
Application：IF, IP-WB, Human, HEK 293 cells.
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Evidence for a role of truncated trkC receptor isoforms in mouse development.
Palko ME, Coppola V, Tessarollo L.
Journal of Neuroscience 1999 Jan; 19(2):775.
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Differential expression of TrkC catalytic and noncatalytic isoforms suggests that they act independently or in association.
Menn B, Timsit S, Calothy G, Lamballe F.
The Journal of Comparative Neurology 1998 Nov; 401(1):47.
Application：IHC-Fr, IP, WB-Ti, Mouse, Brains, Embryos, Hearts, Kidneys, Livers, Lungs, Muscles, Ovaries, Spleens, Testes, .
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A kinase-deficient TrkC receptor isoform activates Arf6-Rac1 signaling through the scaffold protein tamalin.
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