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Last updated: 2017/4/23
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Ntrk3 polyclonal antibody

  • Catalog # : PAB11334
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Ntrk3.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of mouse Ntrk3.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:5000-1:20000)
    Western Blot (1:1000-1:10000)
    Immunoprecipitation (1 ug)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot using Ntrk3 polyclonal antibody (Cat # PAB11334) to detect endogenous Ntrk3 in mouse cortex lysate (Lane 1). Lane 2 is Ntrk3 knock-out cortex lysate.
    Cell extracts were resolved by electrophoresis and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:6,000 dilution.
    Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot using Ntrk3 polyclonal antibody (Cat # PAB11334) to detect over-expressed Ntrk3 in HEK293 cells (Lane 2, arrowhead). Lane 1 is a non-transfected control.
    Cell extracts were resolved by electrophoresis and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:3,000 dilution.
    Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.
  • Immunoprecipitation
  • Immunoprecipitation
  • Mouse cortex lysate was immunoprecipitated with Ntrk3 polyclonal antibody (Cat # PAB11334) and further blotted with affinity purified anti-Ntrk3.
    Lane 1 is wild-type cortex lysate, Lane 2 is Grasp knock-out cortex lysate, and Lane 3 is Ntrk3 knock-out cortex lysate.
    The membrane was probed with the primary antibody at a 1:6,000 dilution.
    Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • Ntrk3
  • Gene Alias:
  • AW125844,TrkC
  • Gene Description:
  • neurotrophic tyrosine kinase, receptor, type 3
  • Other Designations:
  • OTTMUSP00000023300,OTTMUSP00000023301
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