Grasp polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot using Grasp polyclonal antibody (Cat # PAB11330) to detect over-expressed Grasp in HEK293 cells (lane 2, arrowhead).
Lane 1 shows the non-transfected control.
The primary antibody at a 1 : 2,000 dilution.
Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.
Immunoprecipitation
Mouse brain lysate was immunoprecipitated with Grasp polyclonal antibody (Cat # PAB11330).
A blot was prepared and probed with affinity purified anti-Grasp.
Lane 1 is wild-type brain lysate; Lane 2 is Grasp knock-out brain lysate.
The membrane was probed with the primary antibody at a 1:1,000 dilution.
Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Grasp.
Immunogen
A synthetic peptide corresponding to N-terminus of mouse Grasp.
Host
Rabbit
Reactivity
Mouse, Rat
Specificity
This affinity purified antibody is directed against mouse Tamalin protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:4000-1:20000)
Western Blot (1:500-1:3000)
Immunoprecipitation (1 ug)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot using Grasp polyclonal antibody (Cat # PAB11330) to detect over-expressed Grasp in HEK293 cells (lane 2, arrowhead).
Lane 1 shows the non-transfected control.
The primary antibody at a 1 : 2,000 dilution.
Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.Immunoprecipitation
Mouse brain lysate was immunoprecipitated with Grasp polyclonal antibody (Cat # PAB11330).
A blot was prepared and probed with affinity purified anti-Grasp.
Lane 1 is wild-type brain lysate; Lane 2 is Grasp knock-out brain lysate.
The membrane was probed with the primary antibody at a 1:1,000 dilution.
Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Grasp
Entrez GeneID
56149Protein Accession#
NP_062391;Q9JJA9Gene Name
Grasp
Gene Alias
tamalin
Gene Description
GRP1 (general receptor for phosphoinositides 1)-associated scaffold protein
Gene Ontology
HyperlinkOther Designations
GRP1-associated scaffold protein
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Publication Reference
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Hyperpolarization-activated, cyclic nucleotide-gated HCN2 cation channel forms a protein assembly with multiple neuronal scaffold proteins in distinct modes of protein-protein interaction.
Kimura K, Kitano J, Nakajima Y, Nakanishi S.
Genes to Cells: Devoted to Molecular & Cellular Mechanisms 2004 Jul; 9(7):631.
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Phosphorylation and recruitment of Syk by immunoreceptor tyrosine-based activation motif-based phosphorylation of tamalin.
Hirose M, Kitano J, Nakajima Y, Moriyoshi K, Yanagi S, Yamamura H, Muto T, Jingami H, Nakanishi S.
The Journal of Biological Chemistry 2004 Jul; 279(31):32308.
Application：IP, Rat, Rat brain.
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Tamalin is a scaffold protein that interacts with multiple neuronal proteins in distinct modes of protein-protein association.
Kitano J, Yamazaki Y, Kimura K, Masukado T, Nakajima Y, Nakanishi S.
The Journal of Biological Chemistry 2003 Apr; 278(17):14762.
Application：IP, Monkey, Rat, COS-7 cells, Brain.
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Hyperpolarization-activated, cyclic nucleotide-gated HCN2 cation channel forms a protein assembly with multiple neuronal scaffold proteins in distinct modes of protein-protein interaction.
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