Ubash3b polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Ubash3b.
Immunogen
A synthetic peptide corresponding to C-terminus of mouse Ubash3b.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This affinity purified antibody is directed against mouse Sts-1 protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000-1:10000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot using Ubash3b polyclonal antibody (Cat # PAB11329) shows detection of a band ~70 kDa correspondingto mouse Ubash3b.
Approximately 1.0 ug of recombinant (truncated) Ubash3b-GST was separated by SDS-PAGE and probed with the primary antibody diluted to 0.3 ug/mL .Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — Ubash3b
Entrez GeneID
72828Protein Accession#
Q96IG9(Human);NP_116262(Human)Gene Name
Ubash3b
Gene Alias
2810457I06Rik, BB125008, p70
Gene Description
ubiquitin associated and SH3 domain containing, B
Gene Ontology
HyperlinkGene Summary
O
Other Designations
OTTMUSP00000031634
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Publication Reference
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Suppressors of T-cell receptor signaling Sts-1 and Sts-2 bind to Cbl and inhibit endocytosis of receptor tyrosine kinases.
Kowanetz K, Crosetto N, Haglund K, Schmidt MH, Heldin CH, Dikic I.
The Journal of Biological Chemistry 2004 Jul; 279(31):32786.
Application:WB, Human, Mouse, CHO, HEK 293T cells.
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Regulation of ZAP-70 activation and TCR signaling by two related proteins, Sts-1 and Sts-2.
Carpino N, Turner S, Mekala D, Takahashi Y, Zang H, Geiger TL, Doherty P, Ihle JN.
Immunity 2004 Jan; 20(1):37.
Application:IP, WB, Mouse, Bone marrow, Spleen.
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Identification, cDNA cloning, and targeted deletion of p70, a novel, ubiquitously expressed SH3 domain-containing protein.
Carpino N, Kobayashi R, Zang H, Takahashi Y, Jou ST, Feng J, Nakajima H, Ihle JN.
Molecular and Cellular Biology 2002 Nov; 22(21):7491.
Application:WB-Ce, WB-Ti, Human, Mouse, Monkey, COS, 293, HeLa, 3T3, CHO, MEF, DA3, Jurkat, MOLT4 cells, Spleen , Skel. muscle, Heart, Lung, Kidney, Thymus, Brain, Liver.
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Suppressors of T-cell receptor signaling Sts-1 and Sts-2 bind to Cbl and inhibit endocytosis of receptor tyrosine kinases.
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